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Carbapenem-resistant Klebsiella pneumoniae (CRKP) exhibit significant mortality rates in pediatric clients and typically belong to Global superior-risk clones. This research aimed to analyze the molecular epidemiology and carbapenem resistance mechanisms of K. pneumoniae isolates recovered from pediatric individuals, and correlate them with phenotypical information. Twenty-five CRKP isolates had been determined, and antimicrobial susceptibility was assessed using broth microdilution. Carbapenemase output and β-lactamase genes have been detected by phenotypic and genotypic tests.

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Multilocus sequence typing was done to differentiate the strains and full-genome sequencing was assessed to characterize a different sequence sort. Admission to your intensive care device and the use of catheters were significantly optimistic correlates of CRKP infection, plus the mortality rate was 36%. Practically all isolates showed multidrug-resistant phenotype, and many Regular resistant gene was blaKPC. We observed the dissemination of ST307 and clones belonging to CG258, that are regarded as high hazard. In pediatric individuals, these clones present with superior genomic plasticity, favoring adaptation with the KPC and NDM enzymes to Health care environments.

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